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STEC Confirmation Using ddPCR

STEC, or Shiga toxin-producing Escherichia coli, can cause human food poisoning and is often tested for on produce, meat, and other high-risk foods. Confirmation of STEC traditionally involves identifying specific genes associated with producing Shiga toxins, such as stx1, stx2, and eae. However, conventional culture methods for confirmation take several days and often produce inconclusive results.

In contrast, digital droplet polymerase chain reaction (ddPCR) is a rapid, highly sensitive, and precise method for confirming STEC presence. If your products are tested for STEC, ddPCR offers an excellent solution for confirmation due to its high sensitivity, specificity, and quantitative capabilities. This makes it a valuable tool for food safety testing, outbreak investigation, and other applications where accurate detection of STEC is crucial.

In this 20-minute webinar, Dr. Feifei Han will discuss:
✓ ddPCR technology and its applications
✓ The advantages of ddPCR for STEC confirmation
✓ The testing process using STEC ddPCR
✓ Sample requirements and turnaround time
✓ Result interpretation and implications

Who Should Attend:

QA Personnel

Feifei Han, Ph.D.

As a seasoned laboratory director at AEMTEK, Dr. Han has dedicated over a decade to advancing the company's food testing efforts. She has established herself as a leading authority in foodborne pathogen detection and quality testing. With her technical insight and expertise, Dr. Han guides the laboratory and provides insightful support to clients while overseeing the development of standard operating procedures and analytical activities. Holding a Ph.D. in Food Science from Louisiana State University and a B.S. in Food Science from China Agricultural University, Dr. Han boasts extensive experience working with a wide range of food pathogens, including Salmonella, pathogenic E. coli, Campylobacter, Vibrio, and Listeria monocytogenes. Her research and knowledge have been widely recognized in the industry, evidenced by her numerous peer-reviewed articles in esteemed international journals and presentations at national conferences.

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