qPCR Testing for Indoor Environments
Indoor Environments
qPCR Testing for Indoor Environments
Indoor Environments
Mold Detection Using qPCR DNA-Based Technology
At AEMTEK, we offer advanced qPCR (quantitative polymerase chain reaction) testing services to support indoor environmental professionals in accurately identifying and quantifying fungal contamination. Our laboratory is licensed to use the EPA-patented Mold-Specific Quantitative PCR (MSQPCR) method, including ERMI (Environmental Relative Moldiness Index) analysis.
Why Choose qPCR Testing?
Species-Specific Identification
qPCR allows precise detection and quantification of targeted mold species using DNA-based assays—ideal for cases where species-level identification is critical, such as identifying pathogenic Aspergillus species.
High Sensitivity & Large Volume Sampling
Unlike culture or microscopy methods, qPCR can analyze large air volumes (up to 8000L), offering superior sensitivity even in low-spore environments.
Viable or Non-Viable Spores
Detects DNA from spores, hyphal fragments, and fungal structures regardless of viability—ensuring a comprehensive view of mold contamination.
Our qPCR Testing Services
Air Sampling
We use 3-piece PCR cassettes with polycarbonate filters to collect airborne fungal spores. This method is optimal for:
- Pre- and post-remediation verification
- Pre- and post-remediation verification
- Sensitive environments where lower detection limits are critical
Surface and Swab Sampling
Swabs collected from hard-to-reach or moist areas are analyzed via qPCR for both fungi and bacteria. Ideal for:
- Confirming source contamination
- Assessing remediation effectiveness
- Sampling non-porous or sensitive materials
Dust & ERMI Testing
AEMTEK offers full Environmental Relative Moldiness Index (ERMI) panel testing using the EPA-developed MSQPCR method. ERMI is a powerful tool for:
- Evaluating long-term mold burden
- Assessing mold exposure history
- Supporting health-related investigations (e.g., asthma, allergies)
Bulk Material Testing
Building materials such as drywall, duct liner, and insulation can be tested using qPCR to determine the presence of fungal DNA—especially when viable cultures are difficult to obtain.
What is ERMI?
The Environmental Relative Moldiness Index (ERMI) uses qPCR to quantify 36 fungal species in dust samples—26 species linked to water damage and 10 background species. The resulting ERMI score indicates a property’s relative mold burden compared to thousands of homes nationwide.
- ERMI Value < -4: Low moldiness
- ERMI Value -4 to 5: Moderate moldiness
- ERMI Value > 5: High moldiness
ERMI testing is especially valuable for home assessments, legal investigations, and post-remediation documentation.
Partner with AEMTEK for
Indoor Environment
qPCR Testing
We work closely with indoor air quality professionals, industrial hygienists, and environmental consultants to ensure reliable results and expert support. Our services are backed by:
- Decades of molecular microbiology expertise
- Licensed use of EPA-patented MSQPCR technology
- Flexible turnaround times and easy sample submission
- Responsive technical consultation
Ready to Get Started?
Contact us for sampling supplies or consultation.
Have Questions? View our FAQs.
Why AEMTEK?
Benefit From...
Deep Technical Expertise
Get help solving your most complicated challenges from our team of seasoned industry professionals and Ph.D. scientists. We have experience in every aspect of laboratory testing, from chemistry and microbiology to product formulation and processing technology.
Direct Access to Experts
Gain more confidence in your product and food safety decisions by taking advantage of easy access to our Ph.D. scientists. We make sure our knowledgeable staff is easily accessible to address all your questions and provide advice in a timely manner, so you can do your job more efficiently.
A Partner in Growth
Work with a laboratory that cares about your business. For almost two decades, we have partnered with a range of companies to help solve challenges and support company growth. From early start-ups to established names, AEMTEK tailors it’s services to support your goals.
Reliable & Accurate Results
Breathe easier and sleep more soundly recognizing AEMTEK’s focus on analytical integrity.
We use the latest accredited methods and state-of-the-art equipment to deliver only the most accurate and reliable results to our clients.
Competitive Pricing & Fast TATs
Get results faster at competitive prices. All microbiology samples are prepped immediately upon arrival to ensure the quickest turnaround times possible.
Our chemistry testing has quick standard turnaround times.
Top-of-the-line Customer Care
Experience industry leading customer service. Get prompt replies to your questions whether via email, phone, or through our website chat. Our knowledgeable staff knows the importance of timeliness in the manufacturing industry and is here to address your questions and requests with speed and a smiling face.
Laboratory Accreditations
Get A Quote For Indoor Environment qPCR Testing
Analyses are performed using various rapid and classical methods, including the FDA Bacteriological Analytical Manual (BAM), the Compendium of Methods for the Microbiological Examination of Foods, the APHA Standard Methods for the Examination of Dairy Products, and the AOAC International Official Methods of Analysis. We employ rigid quality assurance guidelines in all testing. We offer modern rapid methods of analysis for reliable, timely results. Reports are electronically generated in a user-friendly language and format. In addition, our scientists are always ready to answer your questions and address your concerns.